Coding

Part:BBa_K3317066:Design

Designed by: Jorge Luis Hernandez Ayala   Group: iGEM19_UANL   (2019-10-17)


ccaS* optimized 2019


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1235
    Illegal SpeI site found at 1330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1235
    Illegal NheI site found at 606
    Illegal SpeI site found at 1330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1235
    Illegal XhoI site found at 2252
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1235
    Illegal SpeI site found at 1330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1235
    Illegal SpeI site found at 1330
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Castillo-Hair, S., Baerman, E., Fujita, M., Igoshin, O., & Tabor, J. (2019). Optogenetic control of Bacillus subtilis gene expression. Nature Communications, 10(1). doi: 10.1038/s41467-019-10906-6

References